Mass scale synthesis of graphene nanosheets using waste cardboard for application in perovskite solar cells and supercapacitors.

Advanced graphene-based materials have been proficiently incorporated into next-generation solar cells and supercapacitors because of their high electrical conductivity, large surface area, excellent charge-transport ability, and exceptional optical properties. Herein, we report the synthesis of graphene nanosheets (GNs) from waste cardboard via pyrolysis, with ethyl alcohol as the growth initiator. Additionally, we demonstrated the use of GNs in energy conversion and storage applications. Using the GN electrode in perovskite solar cells resulted in an excellent power conversion efficiency of ∼10.41 % for an active area of 1 cm2, indicating an enhancement of approximately 27 % compared to conventional electrodes. Furthermore, the GNs were used as active electrode materials in supercapacitors with excellent electrochemical performance and a high gravimetric specific capacitance of 167.5 F/g at a scan rate of 2 mV/s. The developed GNs can be efficiently used for energy storage, conversion, and electrochemical sensing applications.

H-Glass Supported Hybrid Gold Nano-Islands for Visible-Light-Driven Hydrogen Evolution.

Flat panel reactors, coated with photocatalytic materials, offer a sustainable approach for the commercial production of hydrogen (H2) with zero carbon footprint. Despite this, achieving high solar-to-hydrogen (STH) conversion efficiency with these reactors is still a significant challenge due to the low utilization efficiency of solar light and rapid charge recombination. Herein, hybrid gold nano-islands (HGNIs) are developed on transparent glass support to improve the STH efficiency. Plasmonic HGNIs are grown on an in-house developed active glass sheet composed of sodium aluminum phosphosilicate oxide glass (H-glass) using the thermal dewetting method at 550 °C under an ambient atmosphere. HGNIs with various oxidation states (Au0, Au+, and Au-) and multiple interfaces are obtained due to the diffusion of the elements from the glass structure, which also facilitates the lifetime of the hot electron to be ≈2.94 ps. H-glass-supported HGNIs demonstrate significant STH conversion efficiency of 0.6%, without any sacrificial agents, via water dissociation. This study unveils the specific role of H-glass-supported HGNIs in facilitating light-driven chemical conversions, offering new avenues for the development of high-performance photocatalysts in various chemical conversion reactions for large-scale commercial applications.

System of wheat intensification (SWI): Effects on lodging resistance, photosynthetic efficiency, soil biomes, and water productivity.

Intense cultivation with narrow row spacing in wheat, a common practice in the Indo-Gangetic plains of South Asia, renders the crop more susceptible to lodging during physiological maturity. This susceptibility, compounded by the use of traditional crop cultivars, has led to a substantial decline in overall crop productivity. In response to these challenges, a two-year field study on the system of wheat intensification (SWI) was conducted. The study involved three different cultivation methods in horizontal plots and four wheat genotypes in vertical plots, organized in a strip plot design. Our results exhibited that adoption of SWI at 20 cm × 20 cm resulted in significantly higher intercellular CO2 concentration (5.9-6.3%), transpiration rate (13.2-15.8%), stomatal conductance (55-59%), net photosynthetic rate (126-160%), and photosynthetically active radiation (PAR) interception (1.6-25.2%) over the existing conventional method (plant geometry 22.5 cm × continuous plant to plant spacing) of wheat cultivation. The lodging resistance capacity of both the lower and upper 3rd nodes was significantly higher in the SWI compared to other cultivation methods. Among different genotypes, HD 2967 demonstrated the highest recorded value for lodging resistance capacity, followed by HD 2851, HD 3086, and HD 2894. In addition, adoption of the SWI at 20 cm × 20 cm enhanced crop grain yield by 36.9-41.6%, and biological yield by 27.5-29.8%. Significantly higher soil dehydrogenase activity (12.06 µg TPF g-1 soil hr-1), arylsulfatase activity (82.8 µg p-nitro phenol g-1 soil hr-1), alkaline phosphatase activity (3.11 n moles ethylene g-1 soil hr-1), total polysaccharides, soil microbial biomass carbon, and soil chlorophyll content were also noted under SWI over conventional method of the production. Further, increased root volumes, surface root density and higher NPK uptake were recorded under SWI at 20×20 cm in comparison to rest of the treatments. Among the tested wheat genotypes, HD-2967 and HD-3086 had demonstrated notable increases in grain and biological yields, as well as improvements in the photosynthetically active radiation (PAR) and chlorophyll content. Therefore, adoption of SWI at 20 cm ×20 cm (square planting) with cultivars HD 2967 might be the best strategy for enhancing crop productivity and resource-use efficiency under the similar wheat growing conditions of India and similar agro-ecotypes of the globe.

Sulfonated nitrogen and added-sulfur sources influence productivity, quality, and nutrient acquisition of soybean-wheat cropping system.

Soybean-wheat is the predominant cropping system covering >2.5 Mha area in India. The lower productivity of soybean-wheat cropping system (SWCS), remains a serious concern primarily due to inadequate nutrient management. Increasing sulfur (S) deficiency is widespread, especially under oilseed-based cropping system. Hence, to standardize the S requirement through customized fertilization, an experiment was conducted in completely randomized block design (RBD) comprised of 12 nutrient sources, replicated thrice. The study aims to evaluate the agronomic performance of sulfonated nitrogen (SN) in comparison to conventional S nutrient sources in SWCS. The maximum soybean productivity was recorded under NPK + S through 40-0-0-13 (SN1), although NPK + 50% S (15 kg/ha) as basal and 50% (15 kg/ha) as top dressing through SN2 10-0-0-75 produced maximum wheat grain yield. When compared with no nitrogen (control), the application of 30 kg S ha-1 to both crops increased the productivity of the soybean-wheat cropping system up to 39%. The maximum system (SWCS) productivity (8.45 tha-1) was obtained with the application of 50% S as basal and 50% as top dressing (SN2-based), remaining N through urea. The highest sustainable yield index of soybean (SYIS), i.e. 0.90 was under SN1+ remaining N through urea and likewise highest sustainable yield index of wheat (SYIW) was under S splitting. The application of SN also improved the nutrient acquisition and grain quality of soybean and wheat with a positive nutrient balance in the soil. The protein content and yield of soybean and wheat grains also improved. The higher gluten content in wheat grain was produced with 60 kg S ha-1 applied. The agronomic efficiency of N and S (AEN and AEs) were highest under SN1 and SN2, respectively (32.8 kg grain/kg N applied; 15 kg grain/kg S applied) in soybean, however in wheat, S splitting and urea application resulted in highest agronomic efficiency (AEN and AES) of N and S (17.1 kg grain/kg N applied; 22.3 kg grain/kg S applied respectively). Hence splitting of S doses of SN along with urea and recommend P, K was found efficient for the soybean-wheat cropping system.

Changes in microbial community structure and yield responses with the use of nano-fertilizers of nitrogen and zinc in wheat-maize system.

The growing popularity of nano-fertilization around the world for enhancing yield and nutrient use efficiency has been realized, however its influence on soil microbial structure is not fully understood. The purpose of carrying out this study was to assess the combined effect of nano and conventional fertilizers on the soil biological indicators and crop yield in a wheat-maize system. The results indicate that the at par grain yield of wheat and maize was obtained with application of 75% of recommended nitrogen (N) with full dose of phosphorus (P) and potassium (K) through conventional fertilizers along with nano-N (nano-urea) or nano-N plus nano-Zn sprays and N100PK i.e. business as usual (recommended dose of fertilizer). Important soil microbial property like microbial biomass carbon was found statistically similar with nano fertilizer-based management (N75PK + nano-N, and N75PK + nano-N + nano-Zn) and conventional management (N100PK), during both wheat and maize seasons. The experimental data indicated that the application of foliar spray of nano-fertilizers along with 75% N as basal is a sustainable nutrient management approach with respect to growth, yield and rhizosphere biological activity. Furthermore, two foliar sprays of nano-N or nano-N + nano-Zn curtailed N requirement by 25%, furthermore enhanced soil microbial diversity and the microbial community structure. The specific microbial groups, including Actinobacteria, Bacteroidia, and Proteobacteria, were present in abundance and were positively correlated with wheat and maize yield and soil microbial biomass carbon. Thus, one of the best nutrient management approaches for sustaining productivity and maintaining sound microbial diversity in wheat-maize rotation is the combined use of nano-fertilizers and conventional fertilizers.

Tripodal Triazine and 1,8-Naphthalimide-based Small Molecules as Efficient Photocatalysts for Visible-light Oxidative Condensation.

Tripodal donor-acceptor (D-A) small molecules Tr-Np3 and Tr-T-Np3 consisting of triphenyl triazine and 1,8-naphthalimide, without and with a thiophene spacer have been synthesized. Their optical and redox properties were thoroughly investigated along with their utilization as photocatalysts in organic transformations. Compounds Tr-Np3 and Tr-T-Np3 showed broad absorption in the range of 290-480 nm in solutions and 300-510 nm in thin films. These tripodal molecules displayed wide optical bandgaps of (Eg opt ) 3.10 eV and 2.64 eV with very deep-lying HOMO energy levels (-6.60 eV and -6.03 eV) and low-lying LUMO levels (-3.50 eV and -3.40 eV). Appreciable electron mobilities of 5.24×10-4  cm2 /Vs and 6.14×10-4  cm2 /Vs were obtained for compounds Tr-Np3 and Tr-T-Np3 respectively by space-charge limited current (SCLC) measurements. Metal-free tripodal molecules Tr-Np3 and Tr-T-Np3 showed excellent photocatalytic abilities towards condensation of aromatic aldehydes and o-phenylenediamine followed by cyclization under visible light to yield benzimidazole derivatives that are of high medicinal value.

Optimizing polyhalite (POLY-4) use in the maize-wheat system: A comparative case study from upper and Trans Indo-Gangetic plains of India.

Increasing complexity in crop nutrient requirement in intensive crop production systems needs alternate multi-nutrient sources. Polyhalite (POLY-4) which contains 14% K2O along withcalcium (17% CaO), magnesium (6% MgO), and sulfur (19% S) can be a possible recourse in this regard. In maize-wheat systems, it was evaluated for productivity, profitability, nutrient usage, and nutrient use efficiency under Indo-Gangetic plain (IGP) zones for consecutive two years (2018-19 and 2019-20). The results revealed that 150% K through POLY-4 produced the maximum maize grain yield under the Trans Indo-Gangetic plains (TGP). The maize grain yield increased by 20.8% and 26.2% under 100% K (POLY-4) and 150% K (POLY-4) over No-K, respectively. But statistically, 100% K (POLY-4) stands similar with both 150% K (POLY-4) and 150% K through muriate of potash (MOP) and equivalent. The trends were noticed under upper Indo-Gangetic plain zones (UGP) also. Similarly, the maximum wheat grain yield (6.12 and 6.29 t/ha under TGP and UGP, respectively) was obtained under 150% K (POLY-4), and remained statistically at par with 100% K (POLY-4), but significantly higher than 150% K (MOP). Under both agro-ecologies i.e. TGP and UGP, the highest system productivity was obtained with recommended N, P, and 150% K application through POLY-4. The added return over NPK remained highest with 150% K (POLY-4) for both maize and wheat. However, higher partial factor productivity for N and S, agronomic, physiological, and translocation efficiencies were noticed under 150% K (POLY-4), and remained at par with 100% K (POLY-4). Increased system yield, added returns, partial factor productivity, agronomic, physiological, and translocation efficiencies under 100% K through POLY-4 (along with recommended N and P) proved its effectiveness as multi-nutrient source for the maize-wheat system under TGP and UGP.

M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation.

Glutamine synthetases (GS) play central roles in cellular nitrogen assimilation. Although GS active-site formation requires the oligomerization of just two GS subunits, all GS form large, multi-oligomeric machines. Here we describe a structural dissection of the archaeal Methanosarcina mazei (Mm) GS and its regulation. We show that Mm GS forms unstable dodecamers. Strikingly, we show this Mm GS oligomerization property is leveraged for a unique mode of regulation whereby labile Mm GS hexamers are stabilized by binding the nitrogen regulatory protein, GlnK1. Our GS-GlnK1 structure shows that GlnK1 functions as molecular glue to affix GS hexamers together, stabilizing formation of GS active-sites. These data, therefore, reveal the structural basis for a unique form of enzyme regulation by oligomer modulation.

Evaluation of an emergency medicine point-of-care ultrasound curriculum adapted for a resource-limited setting in Guyana.

BACKGROUND: This is a cohort pilot study of senior emergency medicine residents and residency-trained emergency medicine registrars practicing in an urban tertiary academic hospital in Guyana in South America. The primary aim was to assess the effectiveness of the current adapted residency ultrasound training curriculum and guide future ultrasound-specific continuing medical education. Ultrasound image acquisition, interpretation, and integration of ultrasound findings into clinical medical decision-making competency were assessed in a multimodal fashion: a written assessment and a practical assessment. METHODS: This was a cross-sectional cohort pilot study of senior emergency medicine residents and registrars (residency graduates) practicing in Guyana, a low-middle-income country. Ultrasound image acquisition and interpretation competency were assessed in a multimodal fashion: a written assessment and a practical assessment. The results will be reported as simple percentages. Participants will be deemed competent if the combined score is greater than 80% on the assessment. RESULTS: All senior residents and graduates of the residency program were competent in the core point-of-care ultrasound applications. The senior residents averaged 89% proficiency, and the residency graduates subdivided based on years since graduation averaged 87-100% proficiency. The more experienced providers performed better on the practical portion of the evaluation. Evaluating the composite analysis of all the participants revealed the Extended Focused Assessment with Sonography in Trauma (EFAST) exam (96%) and cardiac exam (93%) were done with the most proficiency. CONCLUSION: The assessment results of this pilot study suggest that the current residency ultrasound training curriculum adapted to the resources available is associated with sustained competency after graduation. There was minor attrition of knowledge amongst the senior physicians, but all senior residents and all participating residency graduates were competent in the core ultrasound applications.

Conjoint application of nano-urea with conventional fertilizers: An energy efficient and environmentally robust approach for sustainable crop production.

One of the biggest challenges to be addressed in world agriculture is low nitrogen (N) use efficiency (<40%). To address this issue, researchers have repeatedly underlined the need for greater emphasis on the development and promotion of energy efficient, and environmentally sound novel fertilizers, in addition to improved agronomic management to augment nutrient use efficiency for restoring soil fertility and increasing farm profit. Hence, a fixed plot field experiment was conducted to assess the economic and environmental competency of conventional fertilizers with and without nano-urea (novel fertilizer) in two predominant cropping systems viz., maize-wheat and pearl millet-mustard under semi-arid regions of India. Result indicates that the supply of 75% recommended N with conventional fertilizer along with nano-urea spray (N75PK+nano-urea) reduced the energy requirement by ~8-11% and increased energy use efficiency by ~6-9% over 100% nitrogen through prilled urea fertilizer (business as usual). Furthermore, the application of N75PK+ nano-urea exhibited ~14% higher economic yields in all the crops compared with N50PK+ nano-urea. Application of N75PK+nano-urea registered comparable soil N and dehydrogenase activities (35.8 µg TPF g-1 24 hrs-1 across all crops) over the conventional fertilization (N100PK). This indicates that application of foliar spray of nano-urea with 75% N is a soil supportive production approach. More interestingly, two foliar sprays of nano-urea curtailed nitrogen load by 25% without any yield penalty, besides reducing the greenhouse gases (GHG) emission from 164.2 to 416.5 kg CO2-eq ha-1 under different crops. Therefore, the application of nano-urea along with 75% N through prilled urea is an energy efficient, environmentally robust and economically feasible nutrient management approach for sustainable crop production.

Detection and classification of lung diseases for pneumonia and Covid-19 using machine and deep learning techniques.

Since the arrival of the novel Covid-19, several types of researches have been initiated for its accurate prediction across the world. The earlier lung disease pneumonia is closely related to Covid-19, as several patients died due to high chest congestion (pneumonic condition). It is challenging to differentiate Covid-19 and pneumonia lung diseases for medical experts. The chest X-ray imaging is the most reliable method for lung disease prediction. In this paper, we propose a novel framework for the lung disease predictions like pneumonia and Covid-19 from the chest X-ray images of patients. The framework consists of dataset acquisition, image quality enhancement, adaptive and accurate region of interest (ROI) estimation, features extraction, and disease anticipation. In dataset acquisition, we have used two publically available chest X-ray image datasets. As the image quality degraded while taking X-ray, we have applied the image quality enhancement using median filtering followed by histogram equalization. For accurate ROI extraction of chest regions, we have designed a modified region growing technique that consists of dynamic region selection based on pixel intensity values and morphological operations. For accurate detection of diseases, robust set of features plays a vital role. We have extracted visual, shape, texture, and intensity features from each ROI image followed by normalization. For normalization, we formulated a robust technique to enhance the detection and classification results. Soft computing methods such as artificial neural network (ANN), support vector machine (SVM), K-nearest neighbour (KNN), ensemble classifier, and deep learning classifier are used for classification. For accurate detection of lung disease, deep learning architecture has been proposed using recurrent neural network (RNN) with long short-term memory (LSTM). Experimental results show the robustness and efficiency of the proposed model in comparison to the existing state-of-the-art methods.

Effect of parity on non-esterified fatty acid, oxidant/antioxidant status, and zinc and copper levels around periparturient period in Beetal goats of Himalayan Region.

The study was conducted to evaluate the effect of parity and physiological status on non-esterified fatty acid (NEFA), oxidative stress, and zinc and copper levels among the Beetal breed of goat. Thirty dual-purpose Beetal goats reared under the semi-intensive system were selected and based on parity were divided into three groups with 10 animals each viz. Early parity (EP; ≤2 parity), mid parity (MP; 3-6 parity), and late parity (LP; ≥7 parity). Blood samples were collected 3 weeks and 1 week pre-kidding followed by 1, 2, 4, and 8 weeks post-kidding for the estimation of NEFA, oxidant (malondialdehyde [MDA], antioxidant (superoxide dismutase [SOD], catalase [CAT], glutathione [GSH], glutathione peroxidase [GSH-Px], and glutathione S-transferase [GST]), and zinc and copper levels. Significant (p < 0.01) increase was observed in NEFA and MDA levels as the goats approached kidding and continued till 2 weeks post-kidding in MP and LP and 1 week post-kidding in EP goats. Significant decrease in SOD (p < 0.05), CAT (p < 0.05), GSH-Px (p < 0.01), GSH (p < 0.01), and GST (p < 0.05) activities were observed as goats approached kidding and continued to decrease up to 2 weeks post-kidding. Zinc and copper levels showed a significant decline from 3 weeks pre-kidding to 2 weeks post-kidding in MP and LP and 1 week post-kidding in EP goats. A significant effect of parity was observed on MDA (p < 0.05), GSH (p < 0.05), and GSH-Px (p < 0.05) activities only; however, parity × sampling time interaction was observed in all the parameters. Findings highlight a different metabolic, trace mineral (zinc and copper), and oxidative response around the periparturient period in Beetal goats, with the EP goats, responding first to increased metabolic and oxidative stress and also first to recover from oxidant/antioxidant imbalance.

Post-concussion symptoms 1-year after traumatic brain injury: using the Rivermead Post-concussion Questionnaire to identify predictors of severity.

BACKGROUND: Patients who suffer traumatic brain injury (TBI) often experience a constellation of physical, cognitive, and emotional/behavioral symptoms called "post-concussion symptoms" and subsequent long-term disability. This study aimed to investigate the incidence of persistent post-concussion symptoms and possible predictors of long-term disability focusing on demographic, injury, and psychological factors. It was hoped to identify groups at high risk. METHODS: A prospective cohort of 1322 individuals admitted with TBI were assessed in a specialist neurorehabilitation clinic at 10 weeks and 1-year post injury between August 2011 and July 2015. The outcome (post-concussion symptoms) was measured using the Rivermead Post-concussion Questionnaire (RPQ) at 1-year post injury. RESULTS: At 1 yr, 1131 individuals were identified (>90% follow-up). Over 20% exhibited moderate or severe symptom levels on RPQ. A linear regression model showed that previous psychiatric history, lower Glasgow Coma Scale (GCS), severe CT abnormalities, injury caused by assault, pre-injury unemployment, and inability to return to work at 6 weeks post-injury were associated with worse symptoms at 1 yr. The adjusted R2 of the model was 25.1%. CONCLUSION: These findings confirm the high incidence of post-concussion symptoms at 1 yr and identify certain associated features that increase risk. This may allow targeting of certain groups, e.g., return to work or victims of assault.

Integrated agroforestry systems improve soil carbon storage, water productivity, and economic returns in the marginal land of the semi-arid region.

Environmental crises, land degradation, and frequent crop failure threaten the livelihoods of millions of the populace in the semi-arid agroecosystems. Therefore, different combinations of annual crops with perennial fruit trees were assessed to restore the soil carbon, and enhance farm productivity and profitability in a semi-arid climate. The study hypothesized that the integration of perennial fruit trees with seasonal crops may enhance farm productivity, economic returns, and environmental sustainability. Integration of phalsa (Grewia asiatica) with mung bean (Vigna radiata) - potato (Solanum tuberosum) system recorded the highest system productivity (25.9 Mg/ha) followed by phalsa with cowpea (Vigna unguiculata) -mustard (Brassica juncea) systems (21.2 Mg/ha). However, Karonda (Carissa sp.) with mung bean - potato system recorded maximum net return (3529.1 US$/ha), and water use efficiency (33.0 kg/ha-mm). Concerning the benefit-cost (B:C) ratio, among the agroforestry systems, the karonda + cowpea - mustard system registered a maximum BC ratio (3.85). However, SOC density remained higher (9.10 Mg/ha) under the phalsa + cowpea - mustard and Moringa + mung bean - potato system (9.16 Mg/ha) over other systems. Similarly, phalsa + mung bean - potato system had the highest C sustainability index (27.6), carbon sequestration potential (0.6-0.67 Mg/ha/year), and water use efficiency (33.0 kg/ha-mm). Hence, the study suggested that the integration of short-duration leguminous and oilseeds with fruit trees offer a myriad of benefits and an efficient system for restoring the soil C without compromising the food and livelihood security of the rural populace in semiarid regions.

Dieulafoy Lesion of the Colon: A Rare Finding During Colonoscopy.

Dieulafoy lesions are common dilated submucosal vessels that can present with gastrointestinal (GI) bleeding. These lesions are usually found in the stomach or esophagus and colonic Dieulafoy lesions are very rare. Clinical presentation can vary from mild non-threatening GI bleeding to massive and recurrent hemorrhage. Here, we discuss a case of a 71-year-old female patient who presented with a bright red bleed per rectum. Colonoscopy was performed, which revealed a bleeding Dieulafoy lesion in the descending colon with clotted blood in the transverse and descending colon. In this article, we will also review the literature related to the epidemiology, clinical presentation, diagnosis, and management of Dieulafoy lesions.

A Case of Heterotopic Gastric Tissue in Duodenal Bulb: An Interesting Endoscopic Finding.

Gastric heterotopia (GH) is a rare, congenital condition where gastric tissue is found outside of its normal location in the gastric mucosa. It is usually benign and can be found throughout the gastrointestinal (GI) tract. In the duodenum, it is usually seen as multiple polyps, specifically in the duodenal bulb. Here, we discuss the case of a 67-year-old male patient who presented with hematemesis, melena, and abdominal pain. Esophagogastroduodenoscopy (EGD) and biopsy revealed a mass consisting of heterotopic gastric mucosa along with an esophageal ulcer. In this article, we will discuss the literature related to the clinical presentation, diagnosis, and management of GH.

"Can differences in hospitalised mild traumatic brain injury (mTBI) outcomes at 12 months be predicted?"

OBJECTIVES: To identify risk factors for poor outcome one year post-mild traumatic brain injury (mTBI). DESIGN: This study was a prospective observational study using consecutive adult hospital admissions with mTBI. SUBJECTS: A total of 869 consecutive mTBI patients were enrolled in this study. METHODS: All patients were reviewed by the specialist TBI rehabilitation team at six weeks and one year following mTBI. Demographic and injury data collected included: age, gender, TBI severity and Glasgow Coma Scale (GCS). At twelve months, global outcome was assessed by the Extended Glasgow Outcome Score (GOSE) and participation restriction by the Rivermead Head Injury Follow-up Questionnaire (RHFUQ) via semi-structured interview. An ordinal regression (OR) was used to identify associated factors for poor GOSE outcome and a linear regression for a poor RHFUQ outcome. RESULTS: In the GOSE analysis, lower GCS (p < 0.001), medical comorbidity (p = 0.027), depression (p < 0.001) and male gender (p = 0.008) were identified as risk factors for poor outcome. The RHFUQ analysis identified: lower GCS (p = 0.002), female gender (p = 0.001) and injuries from assault (p = 0.003) were variables associated with worse social functioning at one year. CONCLUSION: mTBI is associated with a significant impact upon the physical health and psychosocial function of affected individuals. The results of this study demonstrate that differences in mTBI outcome can be identified at twelve months post-mTBI and that certain features, particularly GCS, are associated with poorer outcomes.

In silico study on spice-derived antiviral phytochemicals against SARS-CoV-2 TMPRSS2 target.

Corona Virus Disease (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a pandemic that has claimed so far over half a million human life across the globe. Researchers all over the world are exploring various molecules including phytochemicals to get a potential anti-COVID-19 drug. Certain phytochemicals present in some spices are claimed to possess antiviral, anti-bacterial, and anti-fungal properties. Hence, an in-silico study was done by selecting eighteen well reported antiviral phytochemicals from some spices commonly used in Indian kitchen viz. Curcuma longa (Turmeric), Nigella sativa (Black cumin), Piper nigrum (Black pepper), Trachyspermum ammi (Carom) and Zingiber officinale (Ginger) to find out whether they can prevent SARS-CoV-2 infection. Firstly, we predicted the structure of TMPRSS2 (transmembrane protease serine 2), a host protein that truncates spike protein of SARS-CoV-2 thereby facilitating its endocytosis, and then docked against its catalytic domain the selected phytochemicals and camostat (a well-known synthetic inhibitor of TMPRSS2). Thereafter, stability of seven best docked phytochemicals and camostat were scrutinized by Molecular Dynamic Simulation (MDS). MDS analysis indicated bisdemethoxycurcumin (BDMC), carvacrol and thymol as better inhibitors than the camostat due to their stable binding with TMPRSS2 in its oxyanion hole and inducing subtle modification in the spatial arrangement of the catalytic triad residues. Among these three phytochemicals, carvacrol appeared to be the best inhibitor, followed by BDMC, whereas thymol was least effective.

Distribution of Fluoride in Plasma, Brain, and Bones and Associated Oxidative Damage After Induced Chronic Fluorosis in Wistar Rats.

The study was aimed to determine fluoride levels in plasma, brain, and bones of Wistar rats following chronic administration of fluoride at different dose levels and the consequent oxidative damage inflicted in these tissues. Brain histomorphology and bone radiographs were also evaluated to assess the extent of damage in these organs. Eighteen rats were randomly divided into three groups with six animals in each group. Group I served as control and groups II and III received 50 and 100 ppm fluoride in tap water, respectively for 180 days. A dose-dependent rise in the levels of fluoride in plasma, brain, and bones was observed in rats. Significant (P < 0.05) alterations in levels of total thiols, glutathione peroxidase, glutathione reductase, acetylcholinesterase, catalase, superoxide dismutase, lipids, as well as protein peroxidation in blood and brain were observed as compared to control in a dose-dependent manner. Radiological examination of bone revealed thinning of bone cortex with haphazard ossification, reduced bone density, and widening of marrow cavity indicating occurrence of flawed bone remodeling upon chronic fluoride exposure. Improper mineralization in bones of intoxicated rats indirectly reflected reduced bone tensile strength. Moreover, alterations in plasma Ca:P ratio and high levels of fluoride in bone ash indicated that chronic fluoride exposure leads to alterations in the bone matrix further corroborating the radio-graphical findings. Additionally, severe microscopic alterations were recorded in the cerebrum and cerebellum of treated rats which included neuronal necrosis, gliosis, spongiosis, perivascular cuffing, congestion, and hemorrhage which correlated well with oxidative changes induced by fluoride intoxication in the brain tissue of rats.

Vocational Outcomes After Traumatic Brain Injury; Prevalence and Risk Factors After 1 Year in a Multivariable Model.

OBJECTIVE: To determine the prevalence of employment status (ES) or full-time study after traumatic brain injury (TBI) in a representative population and its predictive factors. DESIGN: Prospective cohort study. SETTING: Regional Major Trauma Centre. Participants: In total, 1734 consecutive individuals of working age, admitted with TBI to a Regional Trauma Centre, were recruited and followed up at 8 weeks and 1 year with face-to-face interview. Median age was 37.2 years (17.5-58.2); 51% had mild TBI, and 36.8% had a normal computed tomographic (CT) scan. MAIN OUTCOME MEASURE: Complete or partial/modified return to employment or study as an ordinal variable. RESULTS: At 1 year, only 44.9% returned to full-time work/study status, 28.7% had a partial or modified return, and 26.4% had no return at all. In comparison with status at 6 weeks, 9.9% had lower or reduced work status. Lower ES was associated with greater injury severity, more CT scan abnormality, older age, mechanism of assault, and presence of depression, alcohol intoxication, or a psychiatric history. The multivariable model was highly significant (P < .001) and had a Nagelkerke R2 of 0.353 (35.3%). CONCLUSIONS: Employment at 1 year is poor and changes in work status are frequent, occurring in both directions. While associations with certain features may allow targeting of vulnerable individuals in future, the majority of model variance remains unexplained and requires further investigation.